That's an excellent question! Regarding foundation-less frames, we recommend letting bees form their own comb adhesion naturally. As Michael Bush states, "Waxing wood guides is not only not necessary but I don't recommend it. The wax you put on won't be attached as well as the bees will attach it." ~ http://www.bushfarms.com/beesfoundationless.htm
If you're using foundation-less frames from Bee Thinking, rest assured that our comb guides will be exactly what your bees need to start building their own natural comb. Matt Reed briefly discusses the wedge comb guide from Bee Thinking's frames in this video: https://www.youtube.com/watch?v=Cv-qc2AxCOU
If you're using another style of frame, and you're looking to go foundation-less, you may need to develop a guide to help the bees get started. Again, Michael Bush provides the following guidance: "You can just break out the wedge on a top bar, turn it sideways and glue and nail it on to make a guide. Or put Popsicle sticks or paint sticks in the groove. Or just cut out the old comb in a drawn wax comb and leave a row at the top or all the way around. The main thing is that there is an edge that protrudes, preferably at least 1/4." ~ http://www.bushfarms.com/beesfoundationless.htm
There's also some great additional Q&A for foundation-less frames on the link above. I hope this helps. Let us know if we can provide any further support!